FastQCFastQC Report
Wed 16 Dec 2020
HK3TCAFX2_n01_msh3_MA_strain_s.061.2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3TCAFX2_n01_msh3_MA_strain_s.061.2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1770027
Sequences flagged as poor quality0
Sequence length151
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACATCG12400.025.140778145
ACGGACG17400.022.916346145
ACTATGC9750.017.8459058
CTATACT17650.016.4359364
TGTCTCG19900.016.029926145
ATACGGT12700.015.9894486
CTATGCA10000.015.9497759
TACGGTG9700.015.6956577
GAGTCCG7955.456968E-1215.5029279
ACGGTGT11350.015.3301818
TCTCCGG9200.014.972615145
GCCCACC18150.014.779407145
CCTATAT18700.014.737411
CTTATCG9400.014.654059
GTCCTGC4001.2985844E-414.5047146
ATAGTGC12250.014.2038828
ACTGCCC4101.6064815E-414.1461438
CTAGGAC3609.640489E-414.1018063
GCTGTCG8251.4188117E-1014.06041145
CTGTCTG17100.013.991032145
Produced by FastQC (version 0.11.5)