FastQCFastQC Report
Wed 16 Dec 2020
HK3TCAFX2_n01_msh3_MA_strain_s.060.3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3TCAFX2_n01_msh3_MA_strain_s.060.3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences415258
Sequences flagged as poor quality0
Sequence length151
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGTCC900.00362598432.22198
TAATACG3255.0931703E-1126.7754144
TTAGGAC1907.1294526E-626.7166943
ATACGGT3005.820766E-1026.589476
TCTAAGC1550.00180429123.3924983
TACGGTG2502.2740805E-623.1997687
TTAGGCT1902.1867469E-422.9000244
TAGGCTT1902.1867469E-422.9000245
GATAGCG1902.1903176E-422.8945087
TAGGACT1600.00217010922.6614824
ACTGTCG1600.002173149522.656023145
GTCCTAA1650.002594841621.974771
AATACGG2353.6958692E-521.600735
CTGTGCT2704.4349035E-621.4812689
TATACCG1700.003085649621.3284535
CTCTGTG2053.6617057E-421.21937
TCTCCGG2505.9641887E-520.299797145
TACCGAT2155.0496333E-420.2323577
CTCCTGG2908.23114E-619.9998145
ACGGTGT2908.23114E-619.99988
Produced by FastQC (version 0.11.5)