FastQCFastQC Report
Wed 16 Dec 2020
HK3TCAFX2_n01_msh3_MA_strain_C5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3TCAFX2_n01_msh3_MA_strain_C5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1086313
Sequences flagged as poor quality0
Sequence length151
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGGCC6750.027.925606145
GAGTCCG4750.025.9470719
AGAGTCC4850.023.9172528
TATACCG4053.274181E-1123.2777675
CACATCG11000.022.408834145
TCTCCGG5400.021.481236145
ATACCGT4702.3646862E-1020.0585026
TCCGTGC2000.00791702618.1247928
CTATACT9250.018.0318014
ACTGTCG4506.295886E-817.722021145
CCTGCAC2500.001391029817.4046083
GGAGCAC3052.7422977E-416.6391548
TCTATAC7251.8189894E-1116.0042383
TACGGTG5453.198693E-815.96311957
GTATAGA11250.015.4707611
TGGACAG5304.040303E-715.0511545
ATACTAT26550.014.7496686
CTGTGAC2950.004205920714.7455949
CGAGACC13900.014.60415145
ACTATAC9500.014.50383953
Produced by FastQC (version 0.11.5)