FastQCFastQC Report
Wed 16 Dec 2020
HK3TCAFX2_n01_msh3_MA_strain_C4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3TCAFX2_n01_msh3_MA_strain_C4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences566521
Sequences flagged as poor quality0
Sequence length151
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGCC1100.009668658526.3633338
TACAGGC1701.0306006E-425.587947
CTAAACG1952.6083307E-422.3133434
GACCGTG3706.6356733E-921.553806145
CCCACGG4901.8189894E-1120.714046145
ACAGGCA2455.1094416E-520.7140468
CCGATGT2205.8994896E-419.77259
TACAGTG3301.1363227E-619.7724977
CTCTGTG2608.070148E-519.5190057
TACTGAG2659.3413975E-519.1507237
GCTAAGG1900.00587619919.083783
ACAGTGT2307.956742E-418.9128258
GGCCGAG2307.956742E-418.912825145
TCTATAC3852.3741632E-718.835943
CTATACT5458.54925E-1118.628574
GCGCTTT2359.1777305E-418.5153276
CTAGTAC2801.4219378E-418.1295913
TCTGTGC3252.200454E-517.8459478
GCGAGCG2500.001392492717.399797145
CACATCG3804.37619E-617.170855145
Produced by FastQC (version 0.11.5)