FastQCFastQC Report
Wed 16 Dec 2020
HK3TCAFX2_n01_msh3_MA_strain_C3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3TCAFX2_n01_msh3_MA_strain_C3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences530633
Sequences flagged as poor quality0
Sequence length151
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACGCA1352.1406047E-532.2215929
AGTGGAC1500.0014935224.1661978
TATACGG3508.675124E-820.7236465
TATATAG13100.016.6105585
ATACGGT3956.296299E-616.5264536
ATAGGAC2650.00204985116.4225123
CACATCG5753.4706318E-916.390985145
TACAGTG3153.490177E-416.1183915
TGAGTGT3203.9347462E-415.8665426
GCTTTGT3254.4431907E-415.6150818
TACACTG2800.002960027215.5427345
TTACTCC5654.937101E-815.4051894
TACGGTG3304.989913E-415.3784897
GAGTGTA2850.003340898815.262867
CTATACT4802.3777957E-615.11099154
ACTCCTC3901.0476971E-414.8715058
TATAGGA4953.2733333E-614.6530822
TACACAC3000.00468410314.5065535
TCTATAC3507.769454E-414.5065533
CTAAGGG4502.1746937E-514.499718145
Produced by FastQC (version 0.11.5)