FastQCFastQC Report
Wed 16 Dec 2020
HK3TCAFX2_n01_msh3_MA_strain_A36.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3TCAFX2_n01_msh3_MA_strain_A36.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1773658
Sequences flagged as poor quality0
Sequence length151
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACATCG12950.021.833723145
GAGACCC14650.020.784739145
CTCCGAC7400.018.614649145
GACCAGG9400.018.510422145
GCCCACC22300.016.580524145
GAGCCCC39850.016.37371145
CCAGAGG17250.015.970829145
TCTAGCC4201.132306E-515.5399143
CTGTCTG17750.015.1125145
TATACCG8951.8189894E-1214.5849485
GAGTCCG5556.833925E-714.3692029
TACTATG17100.013.9910647
ACTAGGC4201.9710421E-413.8093639
ACTGTGC6352.0824882E-713.7006278
TATATAC62050.013.557253
ACGGTGT9751.0913936E-1113.38445958
AGAGTCC7055.1250026E-813.3686388
GTATAAT34500.013.2427091
CTATGCA12050.013.236369
CCCACGG13800.013.133904145
Produced by FastQC (version 0.11.5)