FastQCFastQC Report
Wed 16 Dec 2020
HK3TCAFX2_n01_msh3_MA_strain_A35.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3TCAFX2_n01_msh3_MA_strain_A35.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1399939
Sequences flagged as poor quality0
Sequence length151
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTG12750.022.744959145
CACATCG10150.019.285595145
TACGGTG9100.018.3240647
ACGGTGT9100.018.3240648
CCTATAC8300.017.4722673
AGGGGTC5001.1057637E-817.3998939
ATACGGT10750.016.862776
CGGTGTT9500.016.789379
TCTCCGC24000.016.614481145
TATACGG12600.016.1133145
TAGGACT4551.3707722E-615.9362444
GTATATG20700.015.7635881
CACCGTA6006.1281753E-915.7082377
GTATATA53600.015.6930051
TATACAG19450.015.2848655
TAGGGAG5756.171831E-815.1325044
CTATACT16300.015.1247674
GAGCCCC27400.015.082025145
TACACCG4451.958602E-514.66492755
TAGGACC4953.2933658E-614.6484674
Produced by FastQC (version 0.11.5)