FastQCFastQC Report
Wed 16 Dec 2020
HK3TCAFX2_n01_msh3_MA_strain_A34.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3TCAFX2_n01_msh3_MA_strain_A34.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1408771
Sequences flagged as poor quality0
Sequence length151
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGACG13800.017.862078145
CACATCG12450.016.305002145
ACAGTGC5002.0936022E-715.9497848
ACGGTGT8250.015.8179688
TACGCCG3254.438697E-415.6201655
CTCCGAC5654.985486E-815.398023145
CCTATAC7553.45608E-1115.368943
CTAAGGG5756.159098E-815.1350653
ATCTCCC9650.015.025705145
ACTGTCG6301.1676093E-814.960116145
GGTGTAC5354.5105844E-714.9063428
ACTGACC3901.0496012E-414.8715958
GAGTCCG3951.16986506E-414.6833479
CTATACC9450.014.5811824
GTATATA47000.014.3506451
CCCTAAT7606.166374E-1014.3140971
AGTGCCT6702.6262569E-814.0669758
CTGTCTG13950.014.032069145
CTAGGCC10850.014.032069145
TCTATAC12550.013.8687853
Produced by FastQC (version 0.11.5)