FastQCFastQC Report
Wed 16 Dec 2020
HK3TCAFX2_n01_msh3_MA_strain_A33.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3TCAFX2_n01_msh3_MA_strain_A33.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2477208
Sequences flagged as poor quality0
Sequence length151
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACATCG18950.020.276857145
GAGTCCG8900.017.9211859
ACGGACG25200.017.261745145
TACAGGC11050.017.0586667
GCCCACC26450.016.445974145
GAGCCCC46550.016.197489145
CTGTCTG22100.016.074512145
ATACAGG21000.015.8843356
TATACGG18750.015.8567075
GTATATA85400.015.1993711
AGACGGG16600.014.849261145
TCTATAC19200.014.7296973
TTAGGAC12400.014.6200483
TACGGTG13650.014.3405287
TCTCCGG12750.014.215556145
TATATAC94450.014.2036593
CCCACGC37300.014.188877145
ACGGTGT13400.014.0670368
TCTCCGC50600.014.041373145
CTCTGTG14000.013.9820147
Produced by FastQC (version 0.11.5)