FastQCFastQC Report
Wed 16 Dec 2020
HK3TCAFX2_n01_msh3_MA_strain_A32.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3TCAFX2_n01_msh3_MA_strain_A32.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences585667
Sequences flagged as poor quality0
Sequence length151
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGATCG1100.00966837426.3636389
CACATCG5300.023.254717145
ATACGGT3503.4942786E-922.7857136
CTAGACG1650.002599584621.9696984
CCGATAC1700.003091283121.3235283
GTACAGT3157.2711737E-720.7142876
CCAAGCT1750.003656946620.7142854
GTGTCGG1800.004304975720.13889145
TACAGGC1800.004304975720.138897
ACATTGC4001.6281774E-819.93758
ATACAGG4752.728484E-1019.8421066
GTGCTCC1850.005044426319.5945959
ACTGTCG2256.8630284E-419.333332145
ACCGTAC2256.8630284E-419.3333328
AACCTCG1900.00588503819.0789477
TACGGTG3051.2734707E-519.0163947
GAGCCCC16400.019.009146145
CTCCTGG4202.8507202E-818.988094145
CTATAGT5356.548362E-1118.9719644
TCCAGAC2307.956561E-418.9130443
Produced by FastQC (version 0.11.5)