FastQCFastQC Report
Wed 16 Dec 2020
HK3TCAFX2_n01_msh3_MA_strain_A31.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3TCAFX2_n01_msh3_MA_strain_A31.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1304908
Sequences flagged as poor quality0
Sequence length151
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACATCG12000.018.729048145
ATACCGT6001.8189894E-1118.1276636
CTGTCTG12900.016.86036145
CCCACGG7402.5465852E-1115.675575145
CTAGGGT3355.594322E-415.151484
TAGGACT5304.0475243E-715.0493814
ACAGGCC7907.094059E-1114.683452145
CGAGACC14750.014.254148145
GAGTCCG5104.4941735E-614.2155959
GACTGGT4652.968822E-514.0321697
GGAGCGG5709.2282426E-713.991138145
AGGACGG4151.779948E-413.975815145
CATACAC7305.3842086E-913.9061543
TTATCCG5801.1207394E-613.752024
CACGAGC20900.013.528622145
CCAGGCC5901.3609479E-613.516863145
TCTCCGG6452.5173358E-713.488287145
GACTACG4302.405197E-413.4882867
TAGTCCG11950.013.3471969
GTCCCAG3350.00975435912.9869821
Produced by FastQC (version 0.11.5)