FastQCFastQC Report
Wed 16 Dec 2020
HK3TCAFX2_n01_msh3_MA_strain_A30.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3TCAFX2_n01_msh3_MA_strain_A30.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences641427
Sequences flagged as poor quality0
Sequence length151
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCCTA1306.471903E-427.8908671
CCTGTCG2654.5529305E-927.358227145
ACAGCGT2151.8645245E-523.6044248
TACCGAG1600.00217442822.656037
TCAGCGC1650.002599998621.9694848
TCTATAC5051.8189894E-1221.5394823
TCTGTCG2704.4411245E-621.481274145
GTACTGC2455.1015173E-520.7189336
GACTATG2104.3120916E-420.7140857
TATACCG2908.225958E-620.0044845
CACATCG4402.2591848E-919.772535145
TACAGGC2959.554264E-619.6608267
CCGATGT2701.0784828E-418.7961149
CAGCGCT1950.00683832918.5895639
TACAGCG1950.00683832918.5895637
TAGCCTC2000.00790284118.1290655
CTGTGTA3602.613957E-618.1248259
ATACAGG4506.266964E-817.7261966
TCCCGCA2550.001587767417.0626492
TAGCTTG2650.002053600516.4187765
Produced by FastQC (version 0.11.5)