FastQCFastQC Report
Wed 16 Dec 2020
HK3TCAFX2_n01_msh3_MA_strain_A29.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3TCAFX2_n01_msh3_MA_strain_A29.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1142564
Sequences flagged as poor quality0
Sequence length151
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTGCG2903.2870776E-722.499889
CCTATAC4803.1286618E-1019.6378883
ACTGTGC3401.5152837E-619.1910728
ACAGTGC3051.27526455E-519.0162898
ACTGTCG3852.3859138E-718.831066145
TACAGGG3902.732504E-718.5896437
GAGTCCG5001.1050361E-817.3999049
CACATCG8850.017.203297145
CTGTCTG11700.016.730679145
GTATAGG6658.185452E-1116.3554421
TAGGACT4109.016794E-615.9166374
CTATACA7803.6379788E-1215.8032714
CTACACT5152.9227158E-715.4873864
ATACGGG5654.9742084E-815.4001686
ACTATGC5203.266141E-715.3364558
CTATGCA5705.5513738E-815.2630769
ACAGGTC2850.003343735115.2630768
TCCTAAG4802.3917255E-615.1060682
CTAGACA4351.5796531E-515.0018874
GTACTAG4351.5796531E-515.0018871
Produced by FastQC (version 0.11.5)