FastQCFastQC Report
Wed 16 Dec 2020
HK3TCAFX2_n01_msh3_MA_strain_A28.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3TCAFX2_n01_msh3_MA_strain_A28.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences772969
Sequences flagged as poor quality0
Sequence length151
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCCGA1102.429528E-432.968651
TCTGTCG3950.025.695559145
CACATCG6400.023.788467145
TCCGACT1600.002169303822.6659472
TATACCG3253.968853E-822.3172425
GAGACCC7300.021.848768145
ACCGATG2104.3136385E-420.7137688
CTATGGG2205.884709E-419.781192
CTGTCTG8000.019.030775145
CCCTAAG1950.006822312718.59771
TCAGGGG2050.009125917.682486145
ACGGTGT4155.2402174E-717.4694428
GAGCCCC20550.017.286673145
GACCAGG5051.2481905E-817.227291145
CCAGAGG8550.016.958641145
ACTGAGC3002.4164387E-416.9162458
TACTGCG2650.002057537216.4146867
ATCCTGG3103.0924546E-416.3779751
TCCTGGA3554.679735E-516.3450222
TACGGTG4451.0873828E-616.2917277
Produced by FastQC (version 0.11.5)