FastQCFastQC Report
Wed 16 Dec 2020
HK3TCAFX2_n01_msh3_MA_strain_A27.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3TCAFX2_n01_msh3_MA_strain_A27.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1545259
Sequences flagged as poor quality0
Sequence length151
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG7100.024.5093175
CACATCG11900.019.495716145
TGGGTCG3451.7433376E-618.912962145
ATACCGA7200.017.1196466
ACTGTGC4256.7333167E-717.058758
CCGCACA4151.0123238E-515.7243524
ACCGATG6209.455107E-915.2015498
TCTGTCG8259.094947E-1214.93933145
GAGCCCC34150.014.648547145
ATACCGT6451.5905243E-814.613766
CTCCGAC6602.1553205E-814.280242145
GAGACCG22450.014.209293145
TACCGAT8752.3646862E-1114.0856547
CCGATGT6803.1908712E-813.8602359
CCTGTCG9951.8189894E-1213.844163145
CCCTAGC3200.00721018113.5950133
ACGGTGT7055.12282E-813.3687388
CTTAGCC4352.6507862E-413.3345723
TACCGAG4904.859253E-513.316277
TATACTG17000.013.2218155
Produced by FastQC (version 0.11.5)