FastQCFastQC Report
Wed 16 Dec 2020
HK3TCAFX2_n01_msh3_MA_strain_A26.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3TCAFX2_n01_msh3_MA_strain_A26.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1077783
Sequences flagged as poor quality0
Sequence length151
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCACTC2901.1908924E-824.9998578
CGAGACG10850.023.386965145
CTAGGAC2857.0906117E-620.3535923
GGCATGG10200.019.901846145
ACGGTGT5509.640644E-1118.454448
GCTGCAC3151.6817925E-518.4151546
GCACTCT3151.6838096E-518.4125929
CCGCACC4354.2648026E-818.335784
GCCCACC12000.018.124897145
CACATCG8150.017.791307145
TACGGTG5751.8371793E-1017.6520717
ATACGGT6603.6379788E-1217.5781026
ACTGTCG4409.670239E-716.477179145
TCTCCGC18150.015.97787145
TATACCG5904.8930815E-915.9767085
CCCTGCC2750.002635344915.818098
TATACTG11100.015.6777675
CTGTCTG10250.015.560886145
CTGCACT5203.26565E-715.336457
GGCAGGG8055.456968E-1215.310472145
Produced by FastQC (version 0.11.5)