FastQCFastQC Report
Wed 16 Dec 2020
HK3TCAFX2_n01_msh3_MA_strain_A25.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3TCAFX2_n01_msh3_MA_strain_A25.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3295251
Sequences flagged as poor quality0
Sequence length151
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGACG34550.022.45274145
CACATCG25950.020.674166145
TACGGTG18600.017.5401467
ACGGTGT18700.017.4463488
GAGCCCC61150.016.479801145
GCCCACC39850.016.191807145
TATACCG17200.016.021175
TATACAG46800.015.804965
ATACGGT21800.015.3017266
CTGTCTG29300.015.093705145
ATACAGG28200.014.6576676
TATACGG23750.014.6560425
GTATATA105950.014.4417751
TACAGGC14400.014.0970817
TATATAC113750.012.9414733
TCTATAC26350.012.93473
CCCACGC50150.012.866272145
CCTGTCG20050.012.655734145
TGTCTCG36750.012.625724145
CTATGCA27350.012.4587419
Produced by FastQC (version 0.11.5)