FastQCFastQC Report
Wed 16 Dec 2020
HK3TCAFX2_n01_msh3_MA_strain_A24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3TCAFX2_n01_msh3_MA_strain_A24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences693304
Sequences flagged as poor quality0
Sequence length151
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTG7850.026.78336145
ATACGGT3955.875336E-1022.026846
TACGGTG3304.6848072E-821.9696317
ACTGTCG2455.11122E-520.714224145
ACAGCGC2205.900978E-419.7726698
GTATAAC4452.5975169E-919.5519121
CCTGTCG3351.3132594E-619.477552145
ACGGTGT3001.1047516E-519.3332758
TCTGTAC2659.339453E-519.1522673
GTCTAGG2359.191924E-418.5119171
GTCTAGA2359.191924E-418.5119171
TATACGG4354.2615284E-818.33465
TAGGACC2000.007910537518.1262534
CCCCACT2450.0012158517.756334
CGAGACG10300.017.597034145
ATCCGGT2500.001392165917.4012031
CCATGCG3453.6762107E-516.8115449
CCTATAG3052.738818E-416.6404953
TCTGTGC3956.3292173E-616.5189388
GTAGACT2650.002055955816.416234
Produced by FastQC (version 0.11.5)