FastQCFastQC Report
Wed 16 Dec 2020
HK3TCAFX2_n01_msh3_MA_strain_A23.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3TCAFX2_n01_msh3_MA_strain_A23.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences853087
Sequences flagged as poor quality0
Sequence length151
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGGCC5700.020.35082145
CACATCG7350.018.741444145
TAGGACT2851.6319596E-417.8080124
ATACGGT4959.742507E-917.5767386
TACGGTG4205.940983E-717.2618547
ACGGTGT4256.724513E-717.0587758
CTGTCTG8100.017.006124145
TATACGG5602.4483597E-916.8312975
GACTACG2600.001811472716.730727
CCCTAGA2650.002055856616.4169731
CCGGCTC3554.695593E-516.338944
TATACCG3605.2926865E-516.112015
TCTATAC7201.6370905E-1116.112013
TCCCTAT5904.8894435E-915.9764031
GTACTGC3655.9553568E-515.8912986
TCTCCGG3655.9583424E-515.890367145
TCCCGTG3203.9518997E-415.859339
TCCGGCT4151.0116177E-515.7237693
GCTCCCG3706.693046E-515.6756327
GGAACGT3254.4445912E-415.6162566
Produced by FastQC (version 0.11.5)