FastQCFastQC Report
Wed 16 Dec 2020
HK3TCAFX2_n01_msh3_MA_strain_A22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3TCAFX2_n01_msh3_MA_strain_A22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences805431
Sequences flagged as poor quality0
Sequence length151
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTACAC2151.8617187E-523.6102713
CACATCG6800.023.45564145
CCTGTCG4751.2732926E-1121.368202145
GACTGCG1700.003092462721.323317
TCTATAC6550.021.0355433
TATACCG4301.6934791E-920.2373755
GACTGCT3301.1375287E-619.7725247
TACCGTA4402.2610038E-919.7725247
ATACAGG7850.019.3995236
GACCGTG4253.2698154E-818.764515145
CCGAGCT1950.006839835618.5895529
GTACCGC2359.183249E-418.5150456
ATCTCCC7550.018.244844145
CTGGACT2400.001057775218.1293164
AGACTGC2450.001214704517.759336
TACGGTG4506.2898835E-817.7220387
TCTCCGG4104.6149216E-717.682745145
CTATACC5852.3283064E-1017.3545594
ATACCGT5101.4053512E-817.0628856
GTATATA27700.017.0166851
Produced by FastQC (version 0.11.5)