FastQCFastQC Report
Wed 16 Dec 2020
HK3TCAFX2_n01_msh3_MA_strain_A21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3TCAFX2_n01_msh3_MA_strain_A21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences947918
Sequences flagged as poor quality0
Sequence length151
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACGG6600.024.172785
TACGGTG5150.023.9317767
ACGGTGT4850.023.9172638
CTGGTCA3905.020411E-1022.3074479
ATACGGT6400.019.2626826
GTCCCTA2701.0770026E-418.801051
CACATCG8500.017.91157145
GAGCCCC24850.017.504839145
ACTATGC5302.2673703E-816.4149158
GCTGGTC3103.1037463E-416.3707898
CCTATAC4501.2190521E-616.1151853
GTACTAG4151.0099648E-515.7268681
ATATACG13000.015.0615014
CGGTGTT5806.8675945E-814.9998379
CCCACGC18700.014.732459145
GACAGAT5959.389805E-814.6216897
CTTACAC4051.4430286E-414.324613
ATACAGG7656.8030204E-1014.2192816
CTACACC4151.7752267E-413.9794384
GTATATG11600.013.7534781
Produced by FastQC (version 0.11.5)