FastQCFastQC Report
Wed 16 Dec 2020
HK3TCAFX2_n01_msh3_MA_strain_A20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3TCAFX2_n01_msh3_MA_strain_A20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1727853
Sequences flagged as poor quality0
Sequence length151
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGACCC11650.019.913969145
GGACAGT6051.8189894E-1219.1778076
GACCTTA6950.018.7811416
CTATACT16450.017.1922634
ACTATGC10100.016.5097398
TTACTCC16450.016.3106084
ACAGTGC5453.2025127E-815.9631478
GTATATA57800.015.9339251
CACATCG12400.015.786137145
CTGTCTG16350.015.519725145
GCCCACC19650.015.496032145
CCGGTCG3304.997655E-415.378636145
CTATGCA12750.015.3527929
GACCAGG9550.015.183097145
GAGCCCC35100.015.078199145
ATACTAT44150.014.9466896
CCTGTCG9250.014.891746145
GCTGTCG9800.014.7957735145
TACGGTG9400.014.6541117
TACCGCA6451.593071E-814.612267
Produced by FastQC (version 0.11.5)