FastQCFastQC Report
Wed 16 Dec 2020
HK3TCAFX2_n01_msh3_MA_strain_A19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3TCAFX2_n01_msh3_MA_strain_A19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1684595
Sequences flagged as poor quality0
Sequence length151
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGGT10250.020.5139486
TACGGTG9550.018.219837
TATACGG12350.017.6128395
CACATCG13500.017.185122145
TCTCCGG9900.016.843372145
TATACCG7750.016.8401475
GTATATA57700.016.7128661
ACGGTGT10100.016.509848
TCCTACC6851.2914825E-1015.8772672
CTAGGAC3304.993949E-415.3801013
TATATAC61550.015.3140613
ACAGCTA12950.015.1157748
TACAGGC8207.2759576E-1215.0304327
CTGTCTG16950.014.970448145
TACACCG6351.2949386E-814.8437865
TACTCCG4401.761556E-514.8308115
ACTGTCG6902.4410838E-914.710091145
CCTACAC5455.557795E-714.6342763
ATACAGG15950.014.5466976
CCTATAC8008.54925E-1114.5012383
Produced by FastQC (version 0.11.5)