FastQCFastQC Report
Wed 16 Dec 2020
HK3TCAFX2_n01_msh3_MA_strain_A18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3TCAFX2_n01_msh3_MA_strain_A18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1319263
Sequences flagged as poor quality0
Sequence length151
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTAAG2353.7061414E-521.5989111
CTAGGCG6700.020.559599145
GAGTCCG4351.9645086E-919.99999
CACATCG8000.019.9374145
CTAGGCC10000.019.574903145
GGCATGG14000.017.607056145
GTCTAGA4659.143332E-817.1530531
ACCTAGG2550.001589796617.0613251
GCCTTAG5252.0154403E-816.5738581
CCATCCG2650.002058316.4150129
CGAGACG12000.016.312418145
TAGGACT4951.8646824E-716.1128644
CCGGCTC6006.1172614E-915.7100414
TCCCGTG5604.4687113E-815.5356369
GAGCCCC29500.015.482973145
GGTAGTC4301.4180823E-515.1743437
TCCGCAC3355.596003E-415.1509013
GCTCCCG5756.1816536E-815.130367
TAGTCCG14450.015.0518289
CCCTTAG4351.579711E-515.0021991
Produced by FastQC (version 0.11.5)