FastQCFastQC Report
Wed 16 Dec 2020
HK3TCAFX2_n01_msh3_MA_strain_A16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3TCAFX2_n01_msh3_MA_strain_A16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3057656
Sequences flagged as poor quality0
Sequence length151
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACATCG29250.022.307556145
ACGGACG33850.021.846249145
TCTATAC24800.019.5894573
CTGTCTG26700.019.550444145
TATACCG18200.018.3267615
GAGCCCC60000.018.12489145
ATACAGG28700.017.1801246
TATACAG47600.016.1472365
GTATATA104750.015.7134341
CCCACGC48350.015.44458145
TATACGG23150.015.3477375
CTATACC21300.015.3190634
TACAGGC15750.015.1903847
TAGGACT15000.014.9854484
ATCTCCC21450.014.871704145
ACAGGCA17500.014.4999118
GAACAGC28650.014.4261196
GCCCACC34800.014.16658145
ACTGTCG12800.014.16007145
TATATAC115800.013.8383083
Produced by FastQC (version 0.11.5)