FastQCFastQC Report
Wed 16 Dec 2020
HK3TCAFX2_n01_msh3_MA_strain_A15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3TCAFX2_n01_msh3_MA_strain_A15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences613094
Sequences flagged as poor quality0
Sequence length151
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACATCG4500.027.38861145
CTCCTGG4301.6971171E-920.232351145
TCTATAC3651.3546378E-719.8676723
CAACGTC1850.005044986519.5943979
TACAGGC2256.8639976E-419.3331387
CCTGCAC1900.00587737319.0834243
TAGACTG2359.1800565E-418.514985
TCTCCGG2359.195164E-418.510452145
CTAGTAC2851.6287823E-417.8111953
ACACTTG2050.00911017317.6870776
CTAAGCA2550.001587548917.0628264
TGTAACC2550.001587548917.0628263
TAAGCAG4307.5723983E-716.864425
GCTATAC2850.0033360415.2667392
CGAGACG10600.015.047018145
GTAGACT2900.003745408715.003524
GGCTATA2900.003745408715.003521
TGTACTG3901.0458521E-414.8752845
CCCTAAT3000.004691971514.5034011
CGGAGCC5506.150476E-714.499853145
Produced by FastQC (version 0.11.5)