FastQCFastQC Report
Wed 16 Dec 2020
HK3TCAFX2_n01_msh3_MA_strain_A14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3TCAFX2_n01_msh3_MA_strain_A14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences965375
Sequences flagged as poor quality0
Sequence length151
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATATATATACATGAGAAGAACGGCATAGTGCGTGTTTATGCTTAAA12370.1281367344296258No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTACG1958.7405915E-626.030814
CCTACGT2001.06377865E-525.380045
ATACAGG11400.024.8075586
ACAGGCC7150.024.335455145
TACAGGC7300.022.842277
CTATACC6100.021.393269
CAGGCAA8300.020.9636769
TATACAG17100.020.7789825
TCTATAC8800.019.7766533
CTCTGTG6700.019.4774447
CGTATAG8600.019.3893689
GTCTAAC3802.0741209E-719.0827371
GCTGCTC4654.496542E-918.7095188
ATAGTCT10100.018.6632068
CTCCTAC2751.2402525E-418.4582121
TCAGTAC4755.838956E-918.3194273
CCCTTGC2851.6330447E-417.8068668
GTCGTAG2050.00911574817.686441
TCCCCAG2901.862456E-417.5034751
TATACCG5501.9244908E-917.1397695
Produced by FastQC (version 0.11.5)