FastQCFastQC Report
Wed 16 Dec 2020
HK3TCAFX2_n01_msh3_MA_strain_A13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3TCAFX2_n01_msh3_MA_strain_A13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences874886
Sequences flagged as poor quality0
Sequence length151
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTATAC4600.025.2201543
GACCGTG6450.019.108437145
CAGGCAA4705.131369E-918.5105519
TACGGTG4354.2689862E-818.3332467
TCGCACA2000.00791030518.1269874
TATACGG5701.6189006E-1017.808975
CCGCTCT2050.00912576717.6828429
CGGACGG2901.8651018E-417.499918145
ATACCGT5401.5097612E-917.4556166
GACCGGG4607.5800926E-817.336874145
GTACCGA3352.8548731E-517.315336
GTCTACA4307.591425E-716.8623141
ACTGTGC3453.6780424E-516.8115148
CTAGTAC5201.7895218E-816.7326033
TACAGGC4358.5770625E-716.6665887
ACTATGC6154.765752E-1016.5039868
ACTGTCG4451.0875556E-616.292057145
ACAGGCA5352.5444024E-816.2616048
GCCTAAT4058.022458E-616.1128771
CTGGTCA3153.505586E-416.1110349
Produced by FastQC (version 0.11.5)