FastQCFastQC Report
Wed 16 Dec 2020
HK3TCAFX2_n01_msh3_MA_strain_A12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3TCAFX2_n01_msh3_MA_strain_A12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences869402
Sequences flagged as poor quality0
Sequence length151
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACAC1650.00259809321.9733313
CTATACT8400.020.7177124
GAGCTCC1850.005046565619.5944549
CCCTACT1950.00683332718.592824
TCCTAAG2851.6306361E-417.8099632
CCTGTCG4104.615813E-717.6828145
TAGTCCG6603.6379788E-1217.5756329
CACATCG7650.017.058702145
TCTATAC5552.1700544E-916.984793
CCATGTG2600.001811561916.730659
GTATTAG8005.456968E-1215.4087981
TAACTGC4802.3891462E-615.1066656
TATACTA19300.015.0283945
TCCCGTG3456.997603E-414.7100399
CCCTAAT4001.2991428E-414.50239851
CCTATAC4502.1740569E-514.50239853
GTATATG12700.014.2740141
TCTAGGA4652.9617811E-514.034582
TCCTATA7254.8785296E-914.0023162
GACTGTA3650.001071900513.904017
Produced by FastQC (version 0.11.5)