FastQCFastQC Report
Wed 16 Dec 2020
HK3TCAFX2_n01_Federica.Wine.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3TCAFX2_n01_Federica.Wine.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences264121
Sequences flagged as poor quality0
Sequence length151
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGACCC2550.036.960495145
TACACTC1000.006061096729.0052645
GACGGGG1500.001491437324.166475145
TATAAGG2802.3249959E-723.3078022
GACCAGG1902.1873534E-422.894558145
TACCATG1952.6078252E-422.3075187
TACTGTA1952.6078252E-422.3075187
CCAGAGG2653.7627651E-621.886621145
TATAGAG2806.0528364E-620.7180465
ATAGCGT1800.00429430820.1425446
TAATACT4351.9390427E-920.003634
TACAGTT2205.880399E-419.7763162
TAGCACT1850.00503194819.5981524
ACCATGT1850.00503746319.5944428
CAGTGAA2608.049123E-519.5190779
CAAAGCT2256.841009E-419.3368424
CTGTCTG3501.9879044E-618.64271145
GAGCCCC7900.018.354288145
TAACCTT2000.007892800518.128295
TAACAGT2400.001055616418.128294
Produced by FastQC (version 0.11.5)