FastQCFastQC Report
Wed 16 Dec 2020
HK3TCAFX2_n01_Federica.Oak.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3TCAFX2_n01_Federica.Oak.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences201941
Sequences flagged as poor quality0
Sequence length151
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTAAG657.322627E-444.6153831
TAGTCCG1406.5748645E-736.259
ACAGACC800.002032774736.2499968
ACGCTAT900.0036214332.222226
CTATCCT1050.007694126627.6190479
TAGCATG1100.00965366726.3636369
ATAGTCC1701.0273044E-425.5882368
AATAGTC2502.264269E-623.1999997
CCGGGCC2003.091188E-421.75145
CTATAGT1950.006822115318.5897454
GTATGTA2050.0091019217.6829289
GAGACCG3703.3716533E-617.635136145
GAATAAG2500.001388183417.41
ATATTCC2750.002625313615.8181824
ATAGTTC3254.4255724E-415.6153848
AGTCCGG950.00771373915.2631597
TATAAGA3901.0424222E-414.8717952
GAGCCCC5404.94927E-714.768518145
GTATTTG2950.004189859614.7457631
TGTCTCG3000.004684822614.5145
Produced by FastQC (version 0.11.5)