FastQCFastQC Report
Wed 16 Dec 2020
HK3TCAFX2_n01_Federica.F1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3TCAFX2_n01_Federica.F1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences530256
Sequences flagged as poor quality0
Sequence length151
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATATATATACATGAGAAGAACGGCATAGTGCGTGTTTATGCTTAAA5390.10164901481548536No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCTG1606.822732E-527.187181145
TATACCG3652.1827873E-1023.8420815
CATAGCG1600.0021739622.6559837
TCGGAGG1850.00504415119.594364145
TACCGAG1850.00504415119.5943647
TCCATAC3051.2701243E-519.0215533
GCCCTGA1950.00682572618.5947861
ATGCAGT4303.7194695E-818.5515426
ACCGATG2751.2409147E-418.454338
ATACGGC2050.00910667817.6877236
TACACTG2550.001586777517.0634525
ATTACAC3854.941776E-616.952653
CCGATGT3002.4143961E-416.916479
ATACCGA4307.5647586E-716.865046
TCTATAC5252.0045263E-816.5759263
CTATGTG2650.002052324416.4195463
CATACTG2650.002052324416.4195465
TGCAGTC2650.002056209116.4149027
TACCGAT3103.1005344E-416.3707777
TACTGCA4451.0856547E-616.2919457
Produced by FastQC (version 0.11.5)