FastQCFastQC Report
Wed 16 Dec 2020
HK3TCAFX2_n01_Federica.CSS_parent.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3TCAFX2_n01_Federica.CSS_parent.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6562032
Sequences flagged as poor quality0
Sequence length151
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACATCG83150.018.833265145
GTATATA170200.015.8921521
TCTCCGG47750.013.968462145
CCTGTCG48300.013.959502145
CGAGACG170950.013.9527855145
GTATAAT121850.012.7951771
ATACAGG48000.012.6900946
TATATAC182400.012.2845833
CTGTCTG100150.012.234039145
AAGGCGG151750.012.087207145
GCCCACC108100.012.072048145
GAGCCCC292750.011.98623145
CCCACGG73350.011.959676145
ATACTAT119100.011.5682756
ATCTCCC62900.011.526129145
TATACTA119500.011.408195
GTATAGA65550.011.394571
TCTATAC47900.011.2027073
CTAAGGG55600.011.083534145
TATACTT115200.011.0786535
Produced by FastQC (version 0.11.5)