FastQCFastQC Report
Wed 16 Dec 2020
HK3TCAFX2_n01_Federica.A7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3TCAFX2_n01_Federica.A7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences502401
Sequences flagged as poor quality0
Sequence length151
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGTCG1952.6032285E-729.743202145
CCTAACA2252.8034265E-829.0082842
GGCATGG7000.023.821117145
GGAGTGC1550.001807379523.3867918
TACGGTG2502.275723E-623.1996977
CTGTGCG1600.002173819322.6559529
ACGGTGT3106.2320214E-721.0481118
CACATCG4901.8189894E-1120.714016145
ATTACGC2505.9538237E-520.3057983
ACCTAAC1850.005032226419.602141
ATACGGT3001.1010143E-519.3388566
CATAGGT1900.00588434219.07877
CCCACGG3501.995295E-618.642614145
CGAGACG5951.4551915E-1118.277073145
GGCAGGG4003.5552875E-718.124763145
TTACGCT2050.00910527717.6879774
CCTGTCG2500.001392209417.399773145
TATACGG3352.8466517E-517.3183775
TTAATCC3002.4086036E-416.92153
GGGTAGG3052.7386515E-416.639128145
Produced by FastQC (version 0.11.5)