FastQCFastQC Report
Tue 5 Jan 2021
HK3T5AFX2_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3T5AFX2_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16814904
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3179081.8906322629020065No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACGG10550.032.83757870
GATCTAG17000.018.5261869
ATCTAGT17650.016.45590470
CGATCTA19750.016.12386568
CGCCGTA6800.015.43682859
CTGCCGG14250.015.22527970
AGCGATC22200.014.50236366
ACTGTGC6550.014.4265968
TACACTG10250.013.6681165
GCGATCT22800.013.50657667
CTAGTAC5551.8189894E-1212.6394593
GTGTATA6800.012.4044031
GAGCGAT26050.012.35857965
GGGTACA4852.2919266E-1012.3191491
TAGCACT6650.012.1214464
GTGTAAG7250.012.1192441
GTTCTAG7400.011.8735841
CGAGCGG8900.011.79558970
CTGTGCG6251.8189894E-1211.7579149
CGACGAG142850.011.63594870
Produced by FastQC (version 0.11.5)