FastQCFastQC Report
Tue 5 Jan 2021
HK3T5AFX2_n02_DJ78_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3T5AFX2_n02_DJ78_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5756401
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC113800.197692968227891No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG92250.16025638241672185No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGACC14300.020.0642670
TCTGACC30350.018.10032370
TACACTG30600.017.630545
CCGACGA100900.017.6164370
TTATACT25100.017.4544724
TAGACTG13700.017.1324945
TATACTG23200.016.7610555
CTATACT20600.016.6735954
CTACACT29500.016.6332244
TACACAG25100.016.3297125
TAGACAG18450.015.9495815
TATACAG18800.015.8389895
TTACACT27350.015.6341014
GACGAGC57900.015.16842670
TCTACAC32750.014.883743
TAGACCG7300.014.8766525
CGACGAG84250.014.45288970
GTATAAG14200.014.3519621
CTACCCT14500.014.2611464
GCCGACG126100.014.23467670
Produced by FastQC (version 0.11.5)