FastQCFastQC Report
Tue 5 Jan 2021
HK3T5AFX2_n02_DJ77_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3T5AFX2_n02_DJ77_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8558846
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC182130.212797379459801No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG147570.1724181040294451No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACGA225600.019.69673370
CGACGAG175100.017.2046870
GACGAGC121350.017.15563470
GCCGACG299500.016.70585870
CTATACT22150.015.6716964
ACGAGCG74050.014.97834270
TGCCGAC346200.014.76565569
TATACAG29200.014.279855
CGAGCGA61350.013.28834670
TATACTC26350.013.1647955
TTATACT25800.013.0468614
CTGCCGA392500.013.04177868
GTATAGT26550.012.846791
TCTGACG962900.012.83784370
TACACCG21150.012.5910745
CTGACGC784300.012.50015870
TACACTG43050.012.4531115
TCCTATA22500.012.3300112
TAGCCCG6000.012.2638715
GCTGCCG418300.012.15380967
Produced by FastQC (version 0.11.5)