FastQCFastQC Report
Tue 5 Jan 2021
HK3T5AFX2_n02_AA18_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3T5AFX2_n02_AA18_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3497819
Sequences flagged as poor quality0
Sequence length76
%GC49

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG98690.28214724661281787No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC82200.23500358366170462No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGACC40050.047.43955670
GCCGACC33800.038.3026170
CCGACGA319350.024.71812270
CTATACT6350.024.28254
GCTGCCC19900.022.68192570
CGACGAG202050.021.9758770
GACGAGC130100.021.3005770
TAACCCG3900.020.6578435
TATACTC6700.020.3897325
ATCTAGT21500.020.01750670
TTATACT5450.019.9333214
TATACCG4600.019.7987235
TACACCG13100.019.2523125
GCCGACG416600.018.855669
TACTCCG7350.018.5865575
TATACTA4150.018.5693325
ACGAGCG75700.018.25763570
TAAAGGT6200.018.0872574
ACATCTT24700.017.99078470
TGTAATA6300.017.8195512
Produced by FastQC (version 0.11.5)