FastQCFastQC Report
Thu 24 Dec 2020
HK3T5AFX2_n02_AA17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3T5AFX2_n02_AA17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGTTATACCTATAAAAGAAAATATAAAGCTCTAAACGAAACTATATAGC11.7543859649122806No Hit
GTATTATTAAAGATTTAAAAGTAACGTGATTTGGAACTCATCTCATCTAG11.7543859649122806No Hit
TTCGCCAACAATGGATTCGTATCATCAGCCCTAACCTACCGTACTCCCTC11.7543859649122806No Hit
GGCTTAGGCTTAGGCTAAGGCTAAGGCTTTGGCTTAGACTTAGGCTTAGA11.7543859649122806No Hit
ACTTCTAAAAAAGAATTTTCAAATCACTAACTCTCAACAATATACTCGTA11.7543859649122806No Hit
CTTCAACAAATGCCGTTTCAAGAATACGAACAAGTGGCTCCAAAAGTGCA11.7543859649122806No Hit
GACCTATATTTCACAAAAAAATGACGTCTCTGAAAAATTCAACGCAAAAC11.7543859649122806No Hit
GCAACACCCACCCGCCGAGATAAGAGAATTTATTTGAGCAGAGTTAAGCA11.7543859649122806No Hit
TGTACAGATATGACACTAACATAAGAGCCATTGGTGGTGCAGCCGATTTC11.7543859649122806No Hit
GCCACAACCTGTCCTCACATCATCAGTTATCTCAAAAACTCACCTACATC11.7543859649122806No Hit
CATCATAATTGTACACTTAACTTATTCAAGCAATCAAACATCAAGAAAAA11.7543859649122806No Hit
CCCACCAGAGAATCCACCTCGCAAAGCTCCCCGCTACGTCAACTGTCACT11.7543859649122806No Hit
ATCTTAATCAGTTCAATAATTCAAGCTACGTAAATGACAAGAGTCTAATA11.7543859649122806No Hit
GAATTCCCCACCAGCGACTACCTCGAACACCACGACCACGACCACCACAA11.7543859649122806No Hit
CAAAAAATACAAAAAACCACATTACACAATAAATATCCTCAACTACAAAC11.7543859649122806No Hit
ATTGTGTCGCACAATAAACGCAGGCTGTCATGCGGAGAGAGATCGATAGA11.7543859649122806No Hit
CCCACAACATAGTGTCCCAGTCACCTGTCAGCTCCCAGTCAATCAACCTA11.7543859649122806No Hit
GGCATAGGCTTGGGATAAGCCTTAGAATTAGTCATAGGCTTAGTCTTAGC11.7543859649122806No Hit
AAGTGAGATGGATGCATCGATTGATCGACCGCAAAGTGTGGACATCTGTC11.7543859649122806No Hit
ATCTTAGAATTTAATAATTACATGATAAATCTCCTCAAATTGTCAAAACA11.7543859649122806No Hit
NNNNNGAGAGGACGACAATCTGAGCGCGCACGCGAAACGGAGAGAGCGGA11.7543859649122806No Hit
CTTTGATGCTCCGTGGATACAAGTTTAGTGCGAGAGGAGAAGGTGAATAG11.7543859649122806No Hit
GTAGGGTACTTGCACTTCTTGACCGAGACCGTTCGAAAATTCGACTGCAT11.7543859649122806No Hit
CCACGACCCCCCGACCAACCACACGGCGCCAGCCAAGCCGCGACCACACG11.7543859649122806No Hit
AAACAACTCTGAGAACTGCAGACTTCCCAGATCGGGACCTAGATACACTA11.7543859649122806No Hit
CGCCACGCTTGAGCGACACGACGCCGAACGTATCGAGGGCGGCGAACACC11.7543859649122806No Hit
ACGTTAGATGCTGGACAGACTAAAAGATAACTTCACAGTCCAAAACTCTA11.7543859649122806No Hit
CCATAAGCCTAAGCCTAAGAATAAACACAAGCATAAGCCTAAGCCTTAGC11.7543859649122806No Hit
GTACGGACACACTAAAAGTGATCATAATACAAATACGCTAGGAATACTGT11.7543859649122806No Hit
AAGTCAGTACAGAGCACTAGATGGTACAAACACAACAACCACTGTATCAT11.7543859649122806No Hit
AAACAAAAGAATTCGCAAAATAGGCTCAAAAAGCAAACAAACAACACAAA11.7543859649122806No Hit
TTAAGTAAAATTGCCCGAATGTGAAATATAAGTCAAATAGGAAAATCTCC11.7543859649122806No Hit
TAATCGAGATATCGAAGCTCAAAAAAAAGTAACAAAAAACAGCAAACCTT11.7543859649122806No Hit
TGGTACACTAGTTCTATGAGGCTTTGTTATTGTACATCTACTCATCCGTA11.7543859649122806No Hit
CTTAAGCAAAAATCTGAATTACATGAACCAATATAAGTGCTTTCAAATAT11.7543859649122806No Hit
TAAACATTCTTAATCTAAAAGACCACATAAAAAAATTACAAATACTATAA11.7543859649122806No Hit
CTCTCGAAATGTCAACGTTCCAGTTGAGATGACACAACAACTGGCAAGAG11.7543859649122806No Hit
GAGAAAATCTGCATGACAGTTTGAGGAATACTATCGAGAAAAGAAATGAA11.7543859649122806No Hit
GTAGAGGAGGACTGAAAGTCAAAAAAAAAAGTGAACACTTCAAATATCAA11.7543859649122806No Hit
GCCACCGCTGAGTCCTACCCCGTACGCTACTCACTTCTCCTGGCCATTAG11.7543859649122806No Hit
CCCTGAGCCTGAACCACATAAACCCCCACACCCAGAGAAAGTAGCTAATC11.7543859649122806No Hit
CCTCATACCTTACAGCGCATACCGCATTGCATACATCTCATAACCTACAA11.7543859649122806No Hit
CTACTAATCCTTATCCTCTCTTAGATTATCTGAATAAAACATCGATGTAA11.7543859649122806No Hit
AGCCGGCGGAAACTCGCGCACCCTCAGTATATCACTCTATGCATCAGCTC11.7543859649122806No Hit
CACAAATCCTCCTGAAAGCAGAAAAATAGCATAAAAAACTACTACATTCA11.7543859649122806No Hit
CCACAACACCGAACCCCTACATACACACACCAATTTACTCAACCGACTCT11.7543859649122806No Hit
GTGTGAGCAGTTGAACAATCAATACTTGAGGAGATACAATAGCTCGAGCT11.7543859649122806No Hit
GAAAACTGGCCCAACCGATCCATTCGTCAACATAGTCAAAATAACAGCCA11.7543859649122806No Hit
CCATCACTCTGCGAAGTCGTATAAAAATCCATAGCTCCGGACGACCGATG11.7543859649122806No Hit
ACTAAATCGACAGCCTAGACGAGATCCGAAAAGAGCAAAAGTCTCATCTA11.7543859649122806No Hit
CGTCAAAATACTCTAACTAAGCCTACCCCACACAAGAAAAACAATAGTTT11.7543859649122806No Hit
ATAAATTAGATATAAAACTATTATTGATACATATCATAGATATTCTAAAT11.7543859649122806No Hit
AAAATTCAATGTATACATATATAAAGATTTTTAATAAAAACAAATAACAA11.7543859649122806No Hit
GTGGAGAACCATCAGGAGAAGAATGAATACTGAAAGCAAAAATTGACATA11.7543859649122806No Hit
CCTAGGGCCTACATGCAAGACTACATACTCTTACCTACATGCCTACCTAG11.7543859649122806No Hit
CATGAAGACATCTAGGAGTACACTGTGGCATACATCCGATTGCACATTGA11.7543859649122806No Hit
GCCCCGCGCAATTATATCTTCCCCACCATTTTATTTCTTAGTGTAACCTC11.7543859649122806No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCTGA50.070.06
ACAAACG50.070.061
AAGTAGC50.070.039
GAGAAAG50.070.035
AAACCCC50.070.020
AACAAGA50.070.069
AATCTGC50.070.047
CTGAGCC50.070.03
TAAACCC50.070.019
CCCAGAG50.070.031
CACACCC50.070.027
CACATAA50.070.015
GAAACAA50.070.067
AACGAAA50.070.064
AGTAGCT50.070.040
AAACACA50.070.057
AAACAAG50.070.068
ACCACAT50.070.013
AGCTAAT50.070.043
CACAAAC50.070.060
Produced by FastQC (version 0.11.5)