FastQCFastQC Report
Tue 5 Jan 2021
HK3T5AFX2_n02_AA17_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3T5AFX2_n02_AA17_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8982741
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC328640.3658571476122934No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG188320.20964647650422072No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGACC54950.021.77753670
GCCGACC27400.021.32633670
CGACGAG328000.021.19699770
CCGACGA452850.020.90081470
CTATACT23500.020.5815474
GACGAGC214800.019.98759770
TTATACT25550.017.9699614
TCCTATA19500.016.9187562
ACGAGCG127900.016.82499370
CTTATAA34100.016.62305870
CGAGCGA101350.016.46815570
GCCGACG585400.016.06081869
ATCTAGT46700.015.95927170
TATACTG30850.015.669615
CTGTGCG22600.015.6430159
TATACTT26950.015.5975635
GATCTAG49750.014.980947569
GTATAAG18650.014.8886251
ACTGTGC35050.014.3822878
TATACCG13900.014.3646265
Produced by FastQC (version 0.11.5)