FastQCFastQC Report
Tue 5 Jan 2021
HK3T5AFX2_n02_AA16_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3T5AFX2_n02_AA16_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6811945
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC398440.5849137067313374No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG291390.42776328933953517No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATC76420.11218528628754343No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG74030.1086767435732379No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT19900.030.77244860
CGCCGTA20950.029.2299459
CGTATCA23000.027.842262
TCATTAA24750.025.73009366
TGGTCGC23850.025.3812255
ATCTCGG25950.023.73106848
CTATACT21150.023.7026964
TCTCGGT27350.023.41185249
GGTCGCC27100.022.20822756
GTGGTCG27850.021.98708254
CGACGAG370000.021.90126670
CTAGTGT28950.021.75534239
CCGACGA518400.021.73318370
CATTAAA29400.021.66006367
ATCTAGT54400.021.48226270
GATCTCG31000.021.33220347
GACGAGC245350.020.97775770
AGATCTC31500.020.7717546
TATACTC24350.020.4313135
TAGACAG22000.019.906475
Produced by FastQC (version 0.11.5)