FastQCFastQC Report
Tue 5 Jan 2021
HK3T5AFX2_n02_AA14_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3T5AFX2_n02_AA14_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4724355
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC203220.4301539575243605No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG57020.12069372432850622No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGACC35650.046.91758770
TCTGACC39950.037.9261270
ATCTAGT56450.027.77035170
GTATTAA3750.027.1699921
GCTGCCC17500.025.79401870
CGACGAG557400.023.31536770
GATCTAG66650.023.25792169
GACGAGC363300.023.1449270
CCGACGA836400.022.85517370
TAATACT8550.020.4986864
CTATACT11050.019.9848274
CGATCTA81750.019.13317968
CGACGAC51050.018.71260570
TAGACAG15250.018.607135
TCTAGTG34200.018.10983570
GGTACCG8150.018.053275
TAGAACT10500.018.0271284
TATACCA5700.017.8232695
ATTATAC8750.017.6371883
GCCGACG1081750.017.56791769
Produced by FastQC (version 0.11.5)