FastQCFastQC Report
Thu 24 Dec 2020
HK3T5AFX2_n02_AA13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3T5AFX2_n02_AA13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACATACTAATCGAGCCTAACCTCTGAGAGTTTTTAAAGCTCCCTACGCT15.0No Hit
ACAACACACACTCATAACCTCCATCAGTAAACTATGTAAAATTCTCTACC15.0No Hit
GAGAAAAACCAAATCAAATCCAATTCAAAATGTATAACGTGAAAACTTTA15.0No Hit
GGTCTCACCACGATGGGTCTCACCACGATCAGACTCACCACGAAGCCTCT15.0No Hit
TAAATATATTGCTTACCTTCTCCTTAAGCTCGTTGAACTTGCAGGCGATG15.0No Hit
CAGTAAGCAACACTCAGATCATGGGACTCAGACGCGCACAGATCGCATGC15.0No Hit
ATACATATGTATGAACTAACATCTTAACCAATAGGTTAGCCCTTGATCTC15.0No Hit
AGTTTACGCAGAGGTATGTTTGTTTAATTGCAAAGGGGTTTGCGCGGGTC15.0No Hit
CTTCTGCATCTCGCACACACTCACATTATCGGACCGTACGCGGCCACCCC15.0No Hit
GGCATAGGCTTAGGCTTAGGCTTAAGCTTAAGCATACTCATAGGCTTATG15.0No Hit
GGTCTAGCCTATTAAGTTAACTTTAAATACCGTAACACTTGTAAGAAAAT15.0No Hit
GGCTAATATTGATTGATCCATGTGGGAGAAAGTTTTGCATTTTCTTGTTT15.0No Hit
CCATTCGAGTTACGACTACGAGGTGCTCCGTGAGATCCTCTTCGACGGCT15.0No Hit
GATCCCGATATGTAGGTCAGCAGGAGTGATAAGGTTCTGTATAGGTATAG15.0No Hit
TCTCCACACAACACACAGCCAGCCAGTAGCACTGACGAAGGGGTAGAGGC15.0No Hit
GCATTGCCTCGCTCTCGCTCACAGCCATCTCACTCTGGTCTTTTTCCGTC15.0No Hit
GCACGACACTCCACAGTTACGGCGGTCCTATTCCGAATCAACGTGCCGAA15.0No Hit
CGACTCAACATCAGCACGAAGCTGGCGCACTTTTCCATAAGACATTATTC15.0No Hit
CGTTTAGCCTGCGCGGCCACTTTTTCGGGATGGCTGACCATGCTGTTCTA15.0No Hit
GCTTCAAGGCCCGCGAGGGGGCGGGGGTGGGGCCGGCGGGGGGCGGGAGG15.0No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers