FastQCFastQC Report
Tue 5 Jan 2021
HK3T5AFX2_n02_AA13_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3T5AFX2_n02_AA13_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6218795
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC300140.48263369350493146No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG240690.3870363953145264No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG136870.2200908696942092No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACT17800.026.9855294
ATCTAGT66400.025.50423870
CGACGAG563800.025.38243570
GACGAGC353150.024.57126670
CCGACGA822950.022.95487670
GATCTAG74650.022.73267469
GCCGACC18250.022.62320970
CGATCTA81800.020.61748968
ACGAGCG214700.019.75175370
GTATTAA10950.019.58281
AAGACAG24200.017.8055825
GCCGACG1071100.017.69568469
TTACACT22950.017.5689374
GCGATCT96150.017.54055267
TATACCG8400.017.516065
ATACCGT13050.017.443896
TCTATAC18150.017.3984833
TATACTG19650.017.1149275
TAATACT22000.017.0526334
TAGACAG22850.017.017795
Produced by FastQC (version 0.11.5)