FastQCFastQC Report
Thu 24 Dec 2020
HK3T5AFX2_n02_AA07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3T5AFX2_n02_AA07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATCGCACACTACAAAATATATGATTAAAATCATCGGAGCGAATTAGCA12.272727272727273No Hit
ATAAAAACCTAAAAACCTGAAACCCCTACCGCAAAAGTGCACTTCAAAAT12.272727272727273No Hit
GGATAACTTATCTCTATGGCAAAGCATTTTTGACAGTGATGCTTTTTCGG12.272727272727273No Hit
TTCTCATTAATCTACAAAGCGTAGCTAATATCTAGCATAACATTCTGTTT12.272727272727273No Hit
CGACTACAGTAACATCTCTACTGAGCACTCACACTTTGTATGCTACTATC12.272727272727273No Hit
GTTAGACAATTCTAATGATTACCATTATAATTATGAAGATCAGTGAAACA12.272727272727273No Hit
CTTTTCTCCACCGATTATACAATTCTTTCGCCAGTACCTTAGGATCTACT12.272727272727273No Hit
CCTGCATTCATCACTAATTTTCAGAAATTAAAAGTAATTTTTTTTATTTT12.272727272727273No Hit
GGGGCGACATGGACAGCTGAACTAATGTAGACATGTAGATCAATTGACCT12.272727272727273No Hit
CCACGGTACTTGCAAACGGTGTGCCCGGTCTTCACTAACCGTATATGACT12.272727272727273No Hit
GTCTAGGTAGTTTTGATAGTCTAGCTAGAATAGGTAGTCTAGGTAGTCTA12.272727272727273No Hit
GGGTGGGCCACCTCGCCCACCTCAAGCCCGCCCAGCTCTACGGCGGCCAG12.272727272727273No Hit
TCTTAGCCCACTCGATCCCCAGACCTTTAGATTATATGTCTCTCGGTGTA12.272727272727273No Hit
AGTATCTTTTGAGACCGACGCATGTGACAACAAGAAAGCAAAATCTCAGA12.272727272727273No Hit
AACGTATGGTATCACCATTGGCACCGATTAGGGTGAGGCTAGGGTCGCTG12.272727272727273No Hit
GCATTCATACTATAGAGATATAGCGTTAAGCAATCTATGTATTGACAATA12.272727272727273No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC12.272727272727273No Hit
GTAAAAAACTCATAAAATGCTCCAACATTCATATAAAATACAAAAGTGTC12.272727272727273No Hit
GTCCCAGTGTTCAAGTAGTGATCCGACGACATGTCGCTGTTGGATTCAAA12.272727272727273No Hit
CACTTACTAGGCATTCCTCGTTTAAGGGCAATAATTACAATACCCTATCC12.272727272727273No Hit
TATCACCAACTAATAAAAACCTATCTAAACCAAACAAAGAGTCTCCTTCA12.272727272727273No Hit
CCCTCTTGTCATGATTGTTTTGGAAATCAACATACAACATTATTATTATG12.272727272727273No Hit
CTAAAAAACTAAAGACATAAACTGACTCCCATCACTACTTCACTAACTAC12.272727272727273No Hit
CCCTAAACCTAAACCTGATCATACTCATAAGAATACTACAGCTAATAAGA12.272727272727273No Hit
AACCTTAACTTCCCAATAAAATCACAAGTCACATCTACTACTAAAATCGA12.272727272727273No Hit
CTTCAGGAGGATATTCCAGTAAACTTCAAAGCATTGTTCATCGGCAATGG12.272727272727273No Hit
ATCTGACACTTAATTGGGATAAAAGCTGTTTAAAATATGTCATGTAACTT12.272727272727273No Hit
CGATTATGAAAAATATAAATCGTCTCATTCTATCATATGCTCATGAAAAT12.272727272727273No Hit
GAATACAGCTCGCGACAGTCAATGAATCCTTCATAGACAGAGTACCACCG12.272727272727273No Hit
CTTACTAAGCAGTTTCGGGGTCTTTGCTGAGATGGAATAACTGAGAACAG12.272727272727273No Hit
AATTCATCCACATCTTTCATTCTCATGCTCAAGAATCGAACCGCCTATCT12.272727272727273No Hit
AACTTAGCATGCTCGACCGTAGACGTAATTCTCTAGTAGACAAATATAGT12.272727272727273No Hit
GTAAAAAACTAAATATATACTTTATACATACTATAATTGATTAGTATAAT12.272727272727273No Hit
GGCCACACGTTAAGGTGGAATGTGATCACTGTTTAGGTCACACGCACTTG12.272727272727273No Hit
AATCCTAATAACTCTTAAAAAAAAGATGATTACTCATATGAATATTCTTA12.272727272727273No Hit
CTCTATTAAATAACCAATAATATTACTCTCTGTGGCTTACCACTATATTT12.272727272727273No Hit
CGATAATACTTATCCAAATCAACTTTCAATAAAATAACAAATAGTAAAAA12.272727272727273No Hit
ACATGAAAGACTCTCAAAAAAAGCTAAGTTTCAATATATTATATACATGA12.272727272727273No Hit
AGTACTGTAGATGTACTCTAGAAATACTGTAGCATTACTCTATGATTACT12.272727272727273No Hit
GTTTACAACATGCTCACACTTAGAGAATGGGCATCAGGGTCCACAGGTCG12.272727272727273No Hit
CCACGGACCGAGCGGTTCGGAAAGGCTGATACACTCAGCTACGCGTTCCA12.272727272727273No Hit
AAGACTATTAGGTACAGAATCAAAGTTTTACCTGATAGACGGATGGATCG12.272727272727273No Hit
ATCTTAGCCTAACTCTTATGTTAGCATTAGTCCTATCCATACATGAACAT12.272727272727273No Hit
GCATTGTCTTATCGAATTTATCATACAGAAATGCTTATATCTTATCTTTT12.272727272727273No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)