FastQCFastQC Report
Tue 5 Jan 2021
HK3T5AFX2_n02_AA07_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3T5AFX2_n02_AA07_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13090888
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC353810.2702719632159407No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG216230.1651759605612698No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACT45600.019.2211174
TATACTG67000.018.4598185
TACACTG72450.017.6998755
CTACACT67500.017.451754
TATACCG25100.017.309145
GTATAGA41750.016.5060711
GTATTAG32500.016.4438821
CCGACGA185150.016.04446670
TTATACT60800.015.8574224
TTACACT62750.015.7557414
TACACCG35900.015.5178015
TACACAG57000.015.4285955
CTTACAC49750.015.3742733
TCTATAC44700.015.3058793
CGAGCGA59300.015.22321170
TAATACT56950.015.2057094
ACTGTGC54250.015.1640518
GCCGACG222250.015.01923770
TAGACAG46200.015.0158785
TCTACAC73500.014.89355853
Produced by FastQC (version 0.11.5)