FastQCFastQC Report
Tue 5 Jan 2021
HK3T5AFX2_n02_AA05_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3T5AFX2_n02_AA05_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12333155
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC371690.3013746279844857No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG295060.2392412971376748No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACT41050.019.1345624
TACACTG61700.018.5709385
TATACTG52100.017.8901985
CTACACT62200.017.1382664
TAATACT50700.016.39174
TTATACT53600.016.159054
TATACAG45250.016.107055
TAGACTG32450.015.6575775
TACACCG31600.015.4134195
TATACTC50450.014.933055
TAGACAG44450.014.8991425
TAAGGGT26850.014.8882684
CCGACGA111200.014.63070970
GTATAAG31600.014.4685641
GTCCTAT25300.014.4571261
GTATTAC39650.014.3694761
ACCGTGC17950.014.0424958
GTATAGA37600.014.030481
TCTACAC67050.013.9756053
TACCGTG21150.013.9068257
Produced by FastQC (version 0.11.5)