FastQCFastQC Report
Tue 5 Jan 2021
HK3T5AFX2_n02_AA04_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3T5AFX2_n02_AA04_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12234197
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC409110.3343987349557964No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG330360.27002998235192716No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACT36550.018.4193154
CCGACGA199750.016.8503670
TACACCG31300.016.4558875
TAGACAG43100.016.3405655
TATACTG43750.016.2579695
TATACAG40250.015.58243755
CCTATAC35100.015.4954043
CGAGCGA63150.015.45794570
CTACACT62250.015.3773994
CGACGAG173000.015.10759870
TACACTG64150.015.0204955
TTATACT40350.014.8597744
GCCGACG253650.014.64908770
TTACACT51250.014.5044614
TAAGGGT24900.014.2226874
TAATACT43150.014.2205664
GACGAGC120150.014.15250970
TATACTT41450.013.9478655
TCTATAC36500.013.93973
TACACAG54850.013.9260565
Produced by FastQC (version 0.11.5)