FastQCFastQC Report
Tue 5 Jan 2021
HK3T5AFX2_n02_AA03_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3T5AFX2_n02_AA03_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6297744
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG94810.15054597328821243No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATACT19100.015.4178664
GTATTAC15950.014.9832561
TATACAG18600.014.1281335
CCTTATA12100.013.9247532
TCTGACG201850.013.57321370
GTATAAG12950.013.56932451
GTATAAT18950.013.5385341
TCTATAC16900.013.4930213
TATACTG22650.013.4582175
GTATAAA21300.013.1998311
ATACCGG18650.013.13314851
ATACTTA15800.012.8578776
CTGACGC162800.012.85276170
ATCTGAC256800.012.83528570
TAATACT22000.012.7481064
CTATACT18750.012.5271394
TACCGGC19350.012.47721852
TATACTT21650.012.4614715
TACACTG30450.012.3121235
TATACCG11200.012.2006525
Produced by FastQC (version 0.11.5)