FastQCFastQC Report
Tue 5 Jan 2021
HK3T5AFX2_n02_AA02_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3T5AFX2_n02_AA02_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3735801
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC67540.18079121452133023No Hit
AGTCTAGGTAGTCTAGGTAGTCTAGGTAGTCTAGGTAGTCTAGGTAGTCT50340.13475021822629202No Hit
ACCTAGACTACCTAGACTACCTAGACTACCTAGACTACCTAGACTACCTA46640.12484605041863847No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTATA10350.025.087092
GACGAGC96250.022.28443370
CTCCTAT12100.021.7771381
CTATACT15800.021.7401034
TATACTC14100.021.61615
ACGAGCG57600.020.6537270
CCGACGA187450.020.02885470
CGACGAG136250.019.72270870
GGGTGAC9300.019.56761419
GGGGTTA6650.019.4865516
TAGAGTG8850.019.3967935
CTAGCGC7800.019.28849235
ATCTAGT17900.018.9609770
GTCAGGG9950.018.64279212
GCCGACC10150.018.61527870
CGATCTA18300.018.3553268
CCTATAT12750.018.1561413
TACACTG21700.018.0815415
TAGACAG15600.017.7410645
TACTCCG11850.017.43167
Produced by FastQC (version 0.11.5)